Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 2 | XPS survey scans of (A) PCL scaffold; (B) PCL air treated scaffold (PCL Air scaffold); (C) PCL + NH2 + scaffold; (D) PCL+ NH2 + + Heparin scaffold. Carbon was detected at 285 eV, oxygen at 532 eV, nitrogen at 400 eV, and sulphur at 168 eV.

Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

Techniques:

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 3 | NGF and BDNF release from NH2 + + Heparin. (A) NH2 + + Heparin + Immobilised NGF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). NGF at 1 ng/ml in solution in PBS was used as a control (triangles). (B) BDNF release from NH2 + + Heparin + Immobilised BDNF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). BDNF at 1 ng/ml in solution was used as a control (triangles).

Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

Techniques: Control

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 4 | Average neurite length and Schwann cell migration of DRG when cultured on PCL scaffolds, PCL Air scaffolds, PCL + NH2 + scaffolds, PCL + NH2 + + Heparin scaffolds, and PCL + NH2 + + Heparin + Immobilised neurotrophin for 7 days. (A) Average neurite length and (B) Schwann cell migration for when DRG were cultured with NGF. NGF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. NGF was added in solution at 1 µg/ml with control surfaces. (C) Average neurite length and (D) Schwann cell migration for when DRG were cultured with BDNF. BDNF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. BDNF was added in solution at 1 µg/ml with control surfaces. (E) Average neurite length and (F) Schwann cell migration for when DRG were cultured with NGF plus BDNF. NGF plus BDNF were immobilised on the bioactive surfaces at 1 and 100 ng/ml. NGF plus BDNF were added in solution at 1 µg/ml with control surfaces. One-way ANOVA statistical analysis was performed with Tukey procedure of multiple comparisons *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

Techniques: Migration, Cell Culture, Control

Journal: Frontiers in Materials

Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

doi: 10.3389/fmats.2021.734683

Figure Lengend Snippet: FIGURE 5 | DRG seeded on control PCL bioactive scaffolds: PCL scaffold, PCL air treated scaffold (PCL Air scaffold), PCL + NH2 + scaffold, PCL + NH2 + + Heparin scaffold, PCL + NH2 + + Heparin + Immobilised NGF at 1 ng/ml scaffold, PCL + NH2 + + Heparin + Immobilised BDNF at 1 ng/ml scaffold, and PCL air treated scaffold (PCL Air scaffold) with NGF plus BDNF at 1 µg/ml in culture medium. B-III tubulin protein in neurites stained in green. S100β of Schwann cells stained in red. Merged channels are yellow, representing the co-localisation of growing neurites and Schwann cells. Scale bar 500 µm.

Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

Techniques: Control, Staining